Flow cytometry detection of sustained humoral immune response (IgG plus IgA) against native spike glycoprotein in asymptomatic/mild SARS-CoV-2 infection

SARS-CoV-2 is the virus that causes the disease called COVID-19, which has caused the worst pandemic of the century. Both, to know the immunological status of general population and to evaluate the efficacy of the vaccination process that is taking place around the world, serological tests represent a key tool. Classic serological tests, based on colorimetric techniques, such as ELISA or CLIA, continue to be the most widely used option. However, a real improvement in results is still needed. We developed a highly sensitive and specific FCM assay that allows the detection of IgG and IgA antibodies, directed against the native and functional S-protein of SARS-CoV-2 exposed on the membrane of a transfected cell line, up to 8 months after infection.

Automated summary

SARS-CoV-2, the virus responsible for COVID-19, has caused the worst pandemic of the century. Serological tests are crucial for evaluating the efficacy of global vaccination efforts. A highly sensitive and specific FCM assay has been developed to detect antibodies against the SARS-CoV-2 S-protein up to 8 months post-infection.