Comparative analysis of the gut microbiota of mice fed a diet supplemented with raw and cooked beef loin powder

We used 16S ribosomal RNA sequencing to evaluate changes in the gut microbiota of mice fed a diet supplemented with either raw or cooked beef loin powder for 9 weeks. Male BALB/c mice (n=60) were randomly allocated to five groups: mice fed AIN-93G chow (CON), chow containing 5% (5RB) and 10% (10RB) raw beef loin powder, and chow containing 5% (5CB) and 10% (10CB) cooked beef loin powder. Dietary supplementation with both RB and CB increased the relative abundance of Clostridiales compared to the CON diet (p<0.05). Mice fed 10RB showed a significantly higher relative abundance of Firmicutes (p=0.018) and Lactobacillus (p=0.001) than CON mice, and the ratio of Firmicutes/Bacteroidetes showed an increasing trend in the 10RB mice (p>0.05). Mice fed 10CB showed a higher abundance of Peptostreptococcaceae and a lower abundance of Desulfovibrionaceae compared with the CON mice (p<0.05). Genes for glycan biosynthesis, which result in short-chain fatty acid synthesis, were enriched in the CB mice compared to the RB mice, which was correlated to a high abundance of Bacteroides. Overall, dietary RB and CB changed the gut microbiota of mice (p<0.05).

Automated summary

In this study, 16S ribosomal RNA sequencing was used to evaluate the changes in the gut microbiota of mice fed with diet supplemented with raw or cooked beef loin powder. The results showed that both raw and cooked beef loin powder increased the relative abundance of Clostridiales in the gut of mice. Additionally, the cooked beef loin powder showed enrichment of genes for glycan biosynthesis leading to short-chain fatty acid synthesis, correlated to a high abundance of Bacteroides. Overall, both raw and cooked beef loin powder changed the gut microbiota of mice significantly.