Long noncoding RNA CCAT1 rs67085638 SNP contribution to the progression of gastric cancer in a Polish population

The role of the long noncoding RNA CCAT1 NC_000008.10:g.128220661C>T (rs67085638) in the development of colon cancer has been reported. Therefore, we assessed the prevalence of rs67085638 in patients with gastric cancer (GC). We also evaluated the effect of rs67085638 on B-cell-specific Moloney leukaemia virus insertion site 1 (BMI1) transcripts in primary GC and counterpart histopathologically confirmed disease-free margin tissue. Using high-resolution melting analysis, we evaluated rs67085638 frequency in patients with the GC genotype (n=214) and controls (n=502) in a Polish Caucasian population. qRT-PCR was used to determine BMI1 transcripts. We observed the trend of rs67085638 association in all patients with GC (p(trend)=0.028), a strong risk of the GC genotype in male (p(trend)=0.035) but not female (p(trend)=0.747) patients, and the association with non-cardia GC (p(trend)=0.041), tumour stages T3 (p(trend)=0.014) and T4 (p(trend)=0.032), differentiation grading G3 (p(trend)=0.009), lymph node metastasis stage N3 (p(trend)=0.0005) and metastasis stage M0 (p(trend)=0.027). We found that significantly increased BMI1 transcripts were associated with the primary GC genotype classified as grade G3 (p=0.011) and as lymph node metastasis N3 (p=0.010) and counterpart marginal tissues (p=0.026, p=0.040, respectively) from carriers of the T/T versus C/C genotypes. rs67085638 may contribute to increased BMI1 transcripts and the progression and rapid growth of GC.

Automated summary

The study suggests that rs67085638 may play a significant role in gastric cancer, showing associations with clinical pathological parameters such as tumor staging and lymph node metastasis, and potentially leading to increased BMI1 transcripts.