期刊
SCIENTIFIC REPORTS
卷 11, 期 1, 页码 -出版社
NATURE RESEARCH
DOI: 10.1038/s41598-021-91693-3
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资金
- National Natural Science Foundation of China [NSFC32001452]
- Science Technology and Innovation Committee of Shenzhen [GJHZ20190821160401654]
- Program for Scientific Research Innovation Team of Young Scholar in Colleges and Universities of Shandong Province [2019KJE011]
- Hong Kong Research Grant Council [AoE/M-05/12, AoE/M-403/16, GRF 12100318, 12103219, 12103220]
The study reveals that animal U1-70K genes are conserved in gene and protein structures, expression patterns, and splicing conservation, and may play crucial roles in cancers and juvenile development. Animal U1-70Ks display unique characteristics of single copy number and a splicing isoform, suggesting their specific role in the animal kingdom.
Eukaryotic cells can expand their coding ability by using their splicing machinery, spliceosome, to process precursor mRNA (pre-mRNA) into mature messenger RNA. The mega-macromolecular spliceosome contains multiple subcomplexes, referred to as small nuclear ribonucleoproteins (snRNPs). Among these, U1 snRNP and its central component, U1-70K, are crucial for splice site recognition during early spliceosome assembly. The human U1-70K has been linked to several types of human autoimmune and neurodegenerative diseases. However, its phylogenetic relationship has been seldom reported. To this end, we carried out a systemic analysis of 95 animal U1-70K genes and compare these proteins to their yeast and plant counterparts. Analysis of their gene and protein structures, expression patterns and splicing conservation suggest that animal U1-70Ks are conserved in their molecular function, and may play essential role in cancers and juvenile development. In particular, animal U1-70Ks display unique characteristics of single copy number and a splicing isoform with truncated C-terminal, suggesting the specific role of these U1-70Ks in animal kingdom. In summary, our results provide phylogenetic overview of U1-70K gene family in vertebrates. In silico analyses conducted in this work will act as a reference for future functional studies of this crucial U1 splicing factor in animal kingdom.
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