期刊
SCIENTIFIC REPORTS
卷 11, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41598-021-93616-8
关键词
-
资金
- Government Research Budget
- Thailand Research Fund [TRG 5480010]
- Ratchadapisaek Sompote Post-Doctoral Fund, Chulalongkorn University
Psoriasis is a chronic autoimmune skin disease, where microRNA miR-378a may contribute to its pathogenesis by disturbing cell cycle progression and impacting biological mechanisms related to inflammation and tight junctions. Target mRNA analysis identified bone morphogenetic protein 2 as a novel target gene of miR-378a, providing insights into the molecular mechanisms underlying psoriasis.
Psoriasis is a chronic autoimmune skin disease driven by dysregulations at the cellular, genomic and genetic levels. MicroRNAs are key mediators of gene expression regulation. However, how microRNAs control the pathogenesis of psoriasis is still unclear. Here, we reported a significant up-regulation of miR-378a-3p (miR-378a) in skin biopsies from active psoriatic lesions while it was down-regulated after treatment with methotrexate or narrow-band ultraviolet B phototherapy. Using the keratinocyte in vitro model, we showed that miR-378a disturbed the cell cycle progression, causing cell cycle arrest at G1 phase. Transcriptomic analysis of keratinocytes with miR-378a overexpression and depletion revealed several important biological mechanisms related to inflammation and tight junction. Target mRNA transcript assessed by luciferase assay identified bone morphogenetic protein 2 as a novel target gene of miR-378a. These findings offer a mechanistic model where miR-378a contributes to the pathogenesis of psoriasis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据