Refrigeration of eggs influences the virulence of Salmonella Typhimurium

Salmonella Typhimurium is a human pathogen associated with eggs and egg-derived products. In Australia, it is recommended that eggs should be refrigerated to prevent condensation that can enhance bacterial penetration across the eggshell. Except for the United States, the guidelines on egg refrigeration are not prescriptive. In the current study, in-vitro and in-vivo experiments were conducted to understand the role of egg storage temperatures (refrigerated vs ambient) on bacterial load and the virulence genes expression of Salmonella Typhimurium. The in-vitro egg study showed that the load of Salmonella Typhimurium significantly increased in yolk and albumen stored at 25 degrees C. The gene expression study showed that ompR, misL, pefA, spvA, shdA, bapA, and csgB were significantly up-regulated in the egg yolk stored at 5 degrees C and 25 degrees C for 96 h; however, an in-vivo study revealed that mice infected with egg yolk stored at 25 degrees C, developed salmonellosis from day 3 post-infection (p.i.). Mice fed with inoculated egg yolk, albumen, or eggshell wash stored at refrigerated temperature did not show signs of salmonellosis during the period of the experiment. Data obtained in this study highlighted the importance of egg refrigeration in terms of improving product safety.

Automated summary

This study investigated the impact of egg storage temperatures on Salmonella Typhimurium, with results indicating a significant increase in bacterial load and up-regulation of relevant genes when eggs were stored at 25 degrees Celsius. In-vivo experiments showed that mice infected with eggs stored at higher temperatures developed salmonellosis, highlighting the importance of refrigeration in improving product safety.