期刊
SCIENTIFIC REPORTS
卷 11, 期 1, 页码 -出版社
NATURE RESEARCH
DOI: 10.1038/s41598-021-94282-6
关键词
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资金
- Larry L. Hillblom Foundation
- Dr. Miriam and Sheldon G. Adelson Medical Research Foundation
- NIH [U54 NS100717]
- Rainwater Charitable Foundation
This study developed a non-invasive method to detect synaptic relationships among neurons from in vitro networks, using extracellular action potential propagation to identify short latency spiking relationships between neurons. The method allows for assembling a functional subset of neuronal connectivity in cultures.
We developed a method to non-invasively detect synaptic relationships among neurons from in vitro networks. Our method uses microelectrode arrays on which neurons are cultured and from which propagation of extracellular action potentials (eAPs) in single axons are recorded at multiple electrodes. Detecting eAP propagation bypasses ambiguity introduced by spike sorting. Our methods identify short latency spiking relationships between neurons with properties expected of synaptically coupled neurons, namely they were recapitulated by direct stimulation and were sensitive to changing the number of active synaptic sites. Our methods enabled us to assemble a functional subset of neuronal connectivity in our cultures.
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