期刊
SCIENTIFIC REPORTS
卷 11, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41598-021-93532-x
关键词
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资金
- China Postdoctoral Science Foundation [2020M682639]
- Special Fund for Science and Technology Innovation Strategy (Construction of High-level Academy of Agricultural Sciences) (Foundation of President of Guangdong Academy of Agricultural Sciences in China) [BZ201909]
- national natural science foundation of China [31471175]
- Modern Agricultural Industry Technology System of Guangdong Province, China [2020KJ105]
- Guangdong Academy of Agricultural Sciences the 13th FiveYear Plan Subject team [201635TD]
- Research and development plan of key fields in Guangdong Province [2018B020202004-11]
- Provincial Rural Revitalization Strategy Project in 2019 [Yuecainong [2019]73]
- Research and development projects in key areas of Guangdong Province [2020B0202090003]
- Guangdong Provincial Key Laboratory [2020B1212060047]
Leaf senescence in rice is a precisely modulated developmental process that affects agronomic traits. This study identified key genes and metabolites involved in the flavonoid pathway in leaf senescence, as well as a MYB transcription factor that suppresses the expression of certain genes. Integrated analysis of transcriptome and metabolome contributes to understanding the molecular mechanism of leaf senescence in rice.
Leaf senescence is one of the most precisely modulated developmental process and affects various agronomic traits of rice. Anti-senescence rice varieties are important for breeding application. However, little is known about the mechanisms underlying the metabolic regulatory process of leaf senescence in rice. In this study, we performed transcriptomic and metabolomic analyses of the flag leaves in Yuenong Simiao (YN) and YB, two indica rice cultivars that differ in terms of their leaf senescence. We found 8524 genes/204 metabolites were differentially expressed/accumulated in YN at 30 days after flowering (DAF) compared to 0 DAF, and 8799 genes/205 metabolites were differentially expressed in YB at 30 DAF compared to 0 DAF. Integrative analyses showed that a set of genes and metabolites involved in flavonoid pathway were significantly enriched. We identified that relative accumulation of PHENYLALANINE AMMONIA-LYASE (PAL), CINNAMATE 4-HYDROXYLASE (C4H), 4-COUMAROYL-COA LIGASE (4CL), CHALCONE SYNTHASE (CHS) and CHALCONE ISOMERASE (CHI) in YN30/0 was higher than that in YB30/0. Three flavonoid derivatives, including phloretin, luteolin and eriodictyol, showed lower abundances in YB than in YN at 30 DAF. We further revealed a MYB transcription factor, which is encoded by OsR498G0101613100 gene, could suppress the expression of CHI and CHS. Our results suggested a comprehensive analysis of leaf senescence in a view of transcriptome and metabolome and would contribute to exploring the molecular mechanism of leaf senescence in rice.
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