期刊
SCIENTIFIC REPORTS
卷 11, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41598-021-97759-6
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资金
- Laboratory Directed Research and Development program of Los Alamos National Laboratory [20180189ER]
- US Department of Energy (DOE) Biological and Environmental Research (BER) Bioimaging Science Program (BSP) under the Biological Systems Science Division (BSSD)
- DOE Office of Science [89233218CNA000001]
- Los Alamos National Laboratory Institutional Computing Program [LA-UR 21-24123]
Calibrating the gain and digital conversion factor of an EMCCD is crucial for accurate photon counting. A new method has been developed to quickly calibrate multiple gain settings of the EMCCD camera, which involves acquiring gain-series calibration data and analyzing images with the EMCCD noise model to estimate camera gain response accurately. Furthermore, a method has been developed to compare results from different calibration approaches, with gain-series calibration outperforming all other methods in the self-consistency test.
Calibration of the gain and digital conversion factor of an EMCCD is necessary for accurate photon counting. We present a new method to quickly calibrate multiple gain settings of an EMCCD camera. Acquiring gain-series calibration data and analyzing the resulting images with the EMCCD noise model more accurately estimates the gain response of the camera. Furthermore, we develop a method to compare the results from different calibration approaches. Gain-series calibration outperforms all other methods in this self-consistency test.
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