Biological autoluminescence for assessing oxidative processes in yeast cell cultures

Nowadays, modern medicine is looking for new, more gentle, and more efficient diagnostic methods. A pathological state of an organism is often closely connected with increased amount of reactive oxygen species. They can react with biomolecules and subsequent reactions can lead to very low endogenous light emission (biological autoluminescence-BAL). This phenomenon can be potentially used as a non-invasive and low-operational-cost tool for monitoring oxidative stress during diseases. To contribute to the understanding of the parameters affecting BAL, we analyzed the BAL from yeast Saccharomyces cerevisiae as a representative eukaryotic organism. The relationship between the BAL intensity and the amount of reactive oxygen species that originates as a result of the Fenton reaction as well as correlation between spontaneous BAL and selected physical and chemical parameters (pH, oxygen partial pressure, and cell concentration) during cell growth were established. Our results contribute to real-time non-invasive methodologies for monitoring oxidative processes in biomedicine and biotechnology.

Automated summary

Modern medicine is seeking new, more gentle, and efficient diagnostic methods, with studies finding a connection between reactive oxygen species and pathological states in organisms. The phenomenon of biological autoluminescence may serve as a non-invasive tool for monitoring oxidative stress during diseases. Analysis of yeast helped establish the relationship between reactive oxygen species and autoluminescence intensity, contributing to real-time monitoring of oxidative processes in biomedicine and biotechnology.