4.7 Article

Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae

期刊

SCIENTIFIC REPORTS
卷 11, 期 1, 页码 -

出版社

NATURE PORTFOLIO
DOI: 10.1038/s41598-021-92126-x

关键词

-

资金

  1. Projects SYMBIT [CZ.02.1.01/0.0/0.0/15_003/0000477]
  2. EXPRO (Czech Science Foundation) [20-01331X]
  3. Spanish Government [CGL2016-75694-P]
  4. Ramon y Cajal [RYC-2014-16608]
  5. project e-Infrastruktura CZ [e-INFRA LM2018140]

向作者/读者索取更多资源

Telomerase RNA (TR) plays a crucial role in telomere DNA synthesis and assembly, but its sequence diversity poses challenges in characterizing it. Long telomere units and corresponding TRs were found in certain fungal species. The presence of variable lengths in the annealing sequence of the template region suggests that species with the same telomere sequence may have different TR templates.
Telomerase RNA (TR) carries the template for synthesis of telomere DNA and provides a scaffold for telomerase assembly. Fungal TRs are long and have been compared to higher eukaryotes, where they show considerable diversity within phylogenetically close groups. TRs of several Saccharomycetaceae were recently identified, however, many of these remained uncharacterised in the template region. Here we show that this is mainly due to high variability in telomere sequence. We predicted the telomere sequences using Tandem Repeats Finder and then we identified corresponding putative template regions in TR candidates. Remarkably long telomere units and the corresponding putative TRs were found in Tetrapisispora species. Notably, variable lengths of the annealing sequence of the template region (1-10 nt) were found. Consequently, species with the same telomere sequence may not harbour identical TR templates. Thus, TR sequence alone can be used to predict a template region and telomere sequence, but not to determine these exactly. A conserved feature of telomere sequences, tracts of adjacent Gs, led us to test the propensity of individual telomere sequences to form G4. The results show highly diverse values of G4-propensity, indicating the lack of ubiquitous conservation of this feature across Saccharomycetaceae.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据