期刊
GENERAL AND COMPARATIVE ENDOCRINOLOGY
卷 227, 期 -, 页码 69-76出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygcen.2015.06.009
关键词
cDNA cloning; Gonadotropin-inhibitory hormone (GnIH); Hypothalamus; Neuropeptide; Turtle
资金
- MEXT/JSPS KAKENHI [22687004, 26291066, 18107002, 22132004, 22227002]
- Grants-in-Aid for Scientific Research [26291066, 22687004] Funding Source: KAKEN
Gonadotropin-inhibitory hormone (GnIH) was discovered in 2000 as a novel hypothalamic neuropeptide that inhibited gonadotropin release in the Japanese quail. GnIH and its orthologs have a common C-terminal LPXRFamide (X = L or Q) motif, and have been identified in vertebrates from agnathans to humans, apart from reptiles. In the present study, we characterized a cDNA encoding GnIH orthologs in the brain of the red-eared slider turtle. The deduced precursor protein consisted of 205 amino-acid residues, encoding three putative peptide sequences that included the LPXRFamide motif at their C-termini. In addition, the precursor sequence was most similar to those of avian species. Immunoaffinity purification combined with mass spectrometry confirmed that three mature peptides were produced in the brain. In situ hybridization and immunohistochemistry showed that turtle GnIH-containing cells were restricted to the periventricular hypothalamic nucleus. Immunoreactive fibers were densely distributed in the median eminence. Thus, GnIH and related peptides may act on the pituitary to regulate pituitary hormone release in turtles as well as other vertebrates. (c) 2015 Elsevier Inc. All rights reserved.
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