期刊
SCIENTIFIC REPORTS
卷 11, 期 1, 页码 -出版社
NATURE RESEARCH
DOI: 10.1038/s41598-021-93506-z
关键词
-
资金
- NIH/NCI [U54CA163071, U54CA163068]
- Molecular Genomics and the Analytic Microscopy Core Facilities at the H. Lee Moffitt Cancer Center & Research Institute
- NCI designated Comprehensive Cancer Center [P30-CA076292]
The study found that sustained adrenergic stimulation by norepinephrine has significant effects on ovarian cancer initiation and progression, particularly affecting ovarian surface epithelial cells and fallopian tube cells differently. Mutations in the p53 gene also influence the cellular response to NE stimulation.
Sustained adrenergic stimulation by norepinephrine (NE) contributes to ovarian carcinoma metastasis and impairment of chemotherapy response. Although the effect of sustained NE stimulation in cancer progression is well established, less is known about its role in cancer initiation. To determine the extent to which stress hormones influence ovarian cancer initiation, we conducted a long-term (>3 months;>40 population doublings) experiment in which normal immortalized fallopian tube secretory (iFTSEC283) and ovarian surface epithelial (iOSE11) cell lines and their isogenic pairs containing a p53 mutation (iFTSEC283(p53R175H); iOSE11(p53R175H)), were continuously exposed to NE (100 nM, 1 mu M, 10 mu M). Fallopian tube cells displayed a p53-independent increase in proliferation and colony-forming ability in response to NE, while ovarian surface epithelial cells displayed a p53-independent decrease in both assays. Fallopian tube cells with mutant p53 showed a mild loss of chromosomes and TP53 status was also a defining factor in transcriptional response of fallopian tube cells to long-term NE treatment.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据