期刊
MATERIALS
卷 14, 期 15, 页码 -出版社
MDPI
DOI: 10.3390/ma14154149
关键词
bone regeneration; poly(lactide-co-glycolide); magnesium hydroxide; extracellular matrix; polydeoxyribonucleotide; porous scaffold
类别
资金
- Basic Science Research Program [2020R1A2B5B03002344]
- Bio & Medical Technology Development Program through the National Research Foundation of Korea - Ministry of Science and ICT (MSIT) [2018M3A9E2024579]
- Korea Medical Device Development Fund - Korea government (the Ministry of Science and ICT) [202011A05-05]
- Korea Medical Device Development Fund - Korea government (Ministry of Health & Welfare, Republic of Korea) [202011A05-05]
- Korea Medical Device Development Fund - Korea government (Ministry of Food and Drug Safety) [202011A05-05]
- Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) - Ministry of Health & Welfare, Republic of Korea [HR16C0002]
- Korea Medical Device Development Fund - Korea government (Ministry of Trade, Industry and Energy) [202011A05-05]
The study combined porous PLGA scaffold with magnesium hydroxide and bone-extracellular matrix, as well as incorporated bioactive polydeoxyribonucleotide to create a PMEP scaffold with pro-osteogenic and pro-angiogenic effects. The results showed that the PMEP scaffold had superior biological properties for human bone-marrow mesenchymal stem cells adhesion, proliferation, and osteogenic differentiation.
Current approaches of biomaterials for the repair of critical-sized bone defects still require immense effort to overcome numerous obstacles. The biodegradable polymer-based scaffolds have been required to expand further function for bone tissue engineering. Poly(lactic-co-glycolic) acid (PLGA) is one of the most common biopolymers owing to its biodegradability for tissue regenerations. However, there are major clinical challenges that the byproducts of the PLGA cause an acidic environment of implanting site. The critical processes in bone repair are osteogenesis, angiogenesis, and inhibition of excessive osteoclastogenesis. In this study, the porous PLGA (P) scaffold was combined with magnesium hydroxide (MH, M) and bone-extracellular matrix (bECM, E) to improve anti-inflammatory ability and osteoconductivity. Additionally, the bioactive polydeoxyribonucleotide (PDRN, P) was additionally incorporated in the existing PME scaffold. The prepared PMEP scaffold has pro-osteogenic and pro-angiogenic effects and inhibition of osteoclast due to the PDRN, which interacts with the adenosine A(2A) receptor agonist that up-regulates expression of vascular endothelial growth factor (VEGF) and down-regulates inflammatory cytokines. The PMEP scaffold has superior biological properties for human bone-marrow mesenchymal stem cells (hBMSCs) adhesion, proliferation, and osteogenic differentiation in vitro. Moreover, the gene expressions related to osteogenesis and angiogenesis of hBMSCs increased and the inflammatory factors decreased on the PMEP scaffold. In conclusion, it provides a promising strategy and clinical potential candidate for bone tissue regeneration and repairing bone defects.
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