期刊
GENE
卷 575, 期 2, 页码 559-566出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.gene.2015.09.041
关键词
Transcriptome; Microarray; Sequencing; RNA-seq; Next-generation sequencing; Housekeeping genes; Probe coverage; Gene expression; Gene ontology; Gene structure
资金
- Simon Fraser University
- Stem Cell Network of Canada
- Compute Canada
- Westgrid
- NNSFC (National Natural Science Foundation of China) [21336009]
Microarray (MA) and high-throughput sequencing are two commonly used detection systems for global gene expression profiling. Although these two systems are frequently used in parallel, the differences in their final results have not been examined thoroughly. Transcriptomic analysis of housekeeping (HK) genes provides a unique opportunity to reliably examine the technical difference between these two systems. We investigated here the structure, genome location, expression quantity, microarray probe coverage, as well as biological functions of differentially identified human HK genes by 9 MA and 6 sequencing studies. These in-depth analyses allowed us to discover, for the first time, a subset of transcripts encoding membrane, cell surface and nuclear proteins that were prone to differential identification by the two platforms. We hope that the discovery can aid the future development of these technologies for comprehensive transcriptomic studies. (C) 2015 Elsevier B.V. All rights reserved.
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