Femtosecond diode-based time lens laser for multiphoton microscopy

We demonstrate a near-infrared, femtosecond, diode laser-based source with kW peak power for two-photon microscopy. At a wavelength of 976 nm, the system produces sub-ps pulses operating at a repetition rate of 10 MHz with kilowatt class peak powers suitable for deep tissue two-photon microscopy. The system, integrated with a laser-scanning microscope, images to a depth of 900 mu m in a fixed sample of PLP-eGFP labeled mouse brain tissue. This represents a significant development that will lead to more efficient, compact, and accessible laser sources for biomedical imaging. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

Automated summary

A near-infrared, femtosecond, diode laser-based source with kW peak power for two-photon microscopy was demonstrated, producing sub-ps pulses at a repetition rate of 10 MHz. Integrated with a laser-scanning microscope, the system achieved imaging to a depth of 900 mu m in a fixed sample of mouse brain tissue labeled with PLP-eGFP, marking a significant development in more efficient and compact laser sources for biomedical imaging.