A fast and efficient preparative method for separation and purification of main bioactive xanthones from the waste of Garcinia mangostana L. by high-speed countercurrent chromatography

In this work, we present a preparative elution method by high-speed countercurrent chromatography (Prep-HSCCC) for the separation and isolation of the main bioactive xanthones present in the alcoholic extract of Garcinia mangostana L. agro-industrial waste. The method proved to be quite efficient, operating with a two-phase solvent system consisting of Methanol/w ater/Ethanol/Hexane/methyl tert-butyl ether (6:3:1:6:4v/v), tail-head elution mode, with mobile phase flow of 5 mL/min and rotation speed of 800 rpm. The method demonstrated repeatability (RSD = 3.8-6.5%) and allowed the isolation of a-mangostin and c-mangostin xanthones, both with purities above 93%, in a single step, performed in a time of 35 min. Isolated substances were identified by HPLC-PDA-MS and 1H and 13C NMR spectroscopy. Both xanthones were efficient scavengers of DPPH'Greek ano teleia and ABTS'+ radicals in a concentration-dependent manner with EC50 at 8.43 and 3.31 mg/mL, respectively, for c-mangostin, and 71.10 and 29.33 mg/mL, respectively, for a-mangostin. (c) 2021 The Authors. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Automated summary

The study introduced a highly efficient Prep-HSCCC method for isolating bioactive xanthones from a Garcinia mangostana extract, demonstrating high purity isolation and efficient scavenging of free radicals by the isolated compounds.