期刊
NATURE COMMUNICATIONS
卷 12, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41467-021-25145-x
关键词
-
资金
- JDRF advanced postdoctoral fellowship (JDRF-APF)
- German Research Foundation (DFG)
- European Foundation for the Study of Diabetes (EFSD)
- NIDDK
- JDRF [31-2008-413]
- NIH [2UC4DK098085]
LATS2, a core component of the Hippo signaling pathway, is activated under diabetic conditions and induces beta-cell failure. Inhibiting LATS2 can improve beta-cell viability and insulin secretion, suggesting it as a potential therapeutic target for improving pancreatic beta-cell survival in diabetes.
Diabetes is characterized by dysfunction and loss of beta-cells, and promoting beta-cell survival is of therapeutic interest. Here the authors show that Large-tumor suppressor 2 (LATS2), a core component of the Hippo signaling pathway, induces beta-cell failure through mTORC1 hyperactivation and autophagic flux suppression. Diabetes results from a decline in functional pancreatic beta-cells, but the molecular mechanisms underlying the pathological beta-cell failure are poorly understood. Here we report that large-tumor suppressor 2 (LATS2), a core component of the Hippo signaling pathway, is activated under diabetic conditions and induces beta-cell apoptosis and impaired function. LATS2 deficiency in beta-cells and primary isolated human islets as well as beta-cell specific LATS2 ablation in mice improves beta-cell viability, insulin secretion and beta-cell mass and ameliorates diabetes development. LATS2 activates mechanistic target of rapamycin complex 1 (mTORC1), a physiological suppressor of autophagy, in beta-cells and genetic and pharmacological inhibition of mTORC1 counteracts the pro-apoptotic action of activated LATS2. We further show a direct interplay between Hippo and autophagy, in which LATS2 is an autophagy substrate. On the other hand, LATS2 regulates beta-cell apoptosis triggered by impaired autophagy suggesting an existence of a stress-sensitive multicomponent cellular loop coordinating beta-cell compensation and survival. Our data reveal an important role for LATS2 in pancreatic beta-cell turnover and suggest LATS2 as a potential therapeutic target to improve pancreatic beta-cell survival and function in diabetes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据