期刊
NATURE COMMUNICATIONS
卷 12, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41467-021-24627-2
关键词
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资金
- National Institutes of Health [U01EB026421, R01CA246304, R01CA218356, R01CA253651, R21CA235340, R21CA240887, R01CA255727, R01HD091773]
- Iris Cantor-UCLA Women's Health Center Executive Advisory Board
- NCATS UCLA CTSI Grant [UL1TR000124]
A newly developed microchip technology enables efficient enrichment of circulating trophoblasts from maternal blood for early detection of placenta accreta spectrum (PAS). The researchers identified a high prevalence of circulating trophoblast clusters in PAS patients and constructed a diagnostic model with excellent performance in distinguishing PAS. The combined trophoblast assay shows potential to improve current diagnostic methods for the early detection of PAS.
Placenta accreta spectrum (PAS) is a high-risk obstetrical condition associated with significant morbidity and mortality. Current clinical screening modalities for PAS are not always conclusive. Here, we report a nanostructure-embedded microchip that efficiently enriches both single and clustered circulating trophoblasts (cTBs) from maternal blood for detecting PAS. We discover a uniquely high prevalence of cTB-clusters in PAS and subsequently optimize the device to preserve the intactness of these clusters. Our feasibility study on the enumeration of cTBs and cTB-clusters from 168 pregnant women demonstrates excellent diagnostic performance for distinguishing PAS from non-PAS. A logistic regression model is constructed using a training cohort and then cross-validated and tested using an independent cohort. The combined cTB assay achieves an Area Under ROC Curve of 0.942 (throughout gestation) and 0.924 (early gestation) for distinguishing PAS from non-PAS. Our assay holds the potential to improve current diagnostic modalities for the early detection of PAS.
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