期刊
EXPERIMENTAL AND THERAPEUTIC MEDICINE
卷 22, 期 2, 页码 -出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/etm.2021.10331
关键词
zhike pingchuan granules; bronchial asthma; IL-6; janus kinase 2; STAT3 pathway; inflammation
资金
- National Natural Science Foundation [81574020]
- Traditional Chinese Medicine Scientific Research Special Project of Henan Province [20-21ZY2057]
- Henan Provincial Key Research and Development and Promotion Special Project (Science and Technology Targeted) [212102311143]
The study aimed to investigate the effects of ZKPC on bronchial asthma and its underlying mechanism. The results showed that ZKPC significantly improved lung pathomorphology and reduced the dry/wet ratio. Additionally, ZKPC inhibited inflammation, oxidative stress levels, and cell apoptosis in bronchial asthmatic mice, as well as suppressed the IL-6/JAK2/STAT3 pathway.
The present study aimed to investigate the effects of zhike pingchuan granules (ZKPC) on bronchial asthma and the underlying mechanism. A bronchial asthma mouse model was established by aerosol inhalation of ovalbumin. The changes in lung pathomorphology were observed by hematoxylin and eosin staining. The levels of IL-1 beta, TNF-alpha and IL-6 in bronchoalveolar lavage fluid (BALF) and serum were detected by corresponding ELISA kits. Levels of reactive oxygen species, malondialdehyde and superoxide dismutase in lung tissues were analyzed using corresponding kits. The expression of proteins related to apoptosis and the IL-6/janus kinase 2 (JAK2)/STAT3 pathway was detected by western blot analysis. The results showed that ZKPC significantly restored the dry/wet ratio and alleviated lung pathomorphology of bronchial asthmatic mice. In addition, ZKPC inhibits inflammation, oxidative stress levels and cell apoptosis in bronchial asthmatic mice and also suppressed the IL-6/JAK2/STAT3 pathway. Fedratinib (a JAK2 inhibitor) further strengthened the alleviative effects of ZKPC on bronchial asthma. In conclusion, ZKPC improved bronchial asthma by suppressing the IL-6/JAK2/STAT3 pathway.
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