4.7 Article

Nuclear exosome HMGB3 secreted by nasopharyngeal carcinoma cells promotes tumour metastasis by inducing angiogenesis

期刊

CELL DEATH & DISEASE
卷 12, 期 6, 页码 -

出版社

SPRINGERNATURE
DOI: 10.1038/s41419-021-03845-y

关键词

-

资金

  1. National Natural Science Foundation of China [81972554, 81672682, 81602385]
  2. Clinical Frontier Technology of Jiangsu [BE2017680]
  3. Provincial Natural Science Foundation of Jiangsu, China [BK20201208]
  4. CSCO Clinical Oncology Research Foundation of Beijing [Y-HS2017-074]
  5. Clinical Medical Center of Nantong [HS2016001]
  6. Science and technology project of Nantong [JC2019077]
  7. Scientific Research Project of Nantong Municipal Health Commission [QA2019060]

向作者/读者索取更多资源

High expression of HMGB3 in nasopharyngeal carcinoma is associated with micronuclei formation and metastasis, while HMGB3-containing nEXOs from NPC cells can accelerate angiogenesis and correlate positively with tumor metastasis. This study suggests that nEXO HMGB3 could serve as a significant biomarker for NPC metastasis and provide a basis for anti-angiogenesis therapy in clinical metastasis.
Distant metastasis accompanied by angiogenesis is the main cause of nasopharyngeal carcinoma (NPC)-related death. Nuclear exosomes (nEXOs) are potential tumour biomarkers. High mobility group box 3 (HMGB3), a nuclear protein, is known to be overexpressed in cancers. However, its role in NPC has not been elucidated. Here, we explore for the first time the function of nEXO HMGB3 in tumour angiogenesis involved in NPC metastasis using a series of in vitro experiments with NPC cell lines and clinical specimens and in vivo experiments with tumour xenograft zebrafish angiogenesis model. We found a high expression of HMGB3 in NPC, accompanied by the formation of micronuclei, to be associated with metastasis. Furthermore, the NPC-secreted HMGB3 expression was associated with tumour angiogenesis. Moreover, HMGB3-containing nEXOs, derived from the micronuclei of NPC cells, were ingested by the human umbilical vein endothelial cells (HUVECs), and accelerated angiogenesis in vitro and in vivo. Importantly, western blotting and flow cytometry analysis showed that circulating nEXO HMGB3 positively correlated with NPC metastasis. In summary, nEXO HMGB3 can be a significant biomarker of NPC metastasis and provide a novel basis for anti-angiogenesis therapy in clinical metastasis.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据