Measuring the effect of Mankai® (Wolffia globosa) on the gut microbiota and its metabolic output using an in vitro colon model

Mankai (R) is a cultivated strain of Wolffia globosa an aquatic plant of the family Lemnaceae commonly known as Duckweeds. Recent studies suggest that consumption of a Mankai (R) enriched diet may provide positive health effects by decreasing body weight and improving glucose homeostasis and plasma lipid profiles. However, the effects of Mankai (R) alone on the composition and metabolic output of the human gut microbiota has not been fully investigated. Here, Mankai (R) was digested and fermented in vitro using a batch culture model of the proximal colon. Inulin and cellulose were used as readily and poorly fermentable control fibers respectively. Mankai (R) significantly stimulated the production of phenolic metabolites and short chain fatty acids by the gut microbiota (p<0.05). Three major microbial metabolites, 3-4-hydroxyphenyl propionic acid, 3-3-hydroxyphenyl propanoic acid and protocatechuic acid were significantly increased after 24 h fermentation. Moreover, Mankai (R) treatment lowered the overall microbial diversity (p<0.05), in line with a selective microbiome modulation.

Automated summary

The study found that Mankai (R) significantly stimulated the metabolic activity of the gut microbiota and reduced microbial diversity, indicating its potential impact on health through selective modulation of microbiome composition.