期刊
TICKS AND TICK-BORNE DISEASES
卷 12, 期 5, 页码 -出版社
ELSEVIER GMBH
DOI: 10.1016/j.ttbdis.2021.101782
关键词
Borrelia burgdorferi; Lyme disease; Transwell; Tick saliva; Chemoattractant
资金
- Centers for Disease Control and Prevention
A novel transwell assay was developed to assess the migration of Borrelia burgdorferi s.s. towards Ixodes scapularis salivary gland proteins. Specific proteins in tick saliva gland extract were found to have chemoattractant activity for B. burgdorferi s.s. These findings suggest that further research on tick saliva components may lead to a better understanding of the chemotaxis of B. burgdorferi s.s.
We developed a transwell assay to quantify migration of the Lyme disease agent, Borrelia burgdorferi sensu stricto (s.s.), toward Ixodes scapularis salivary gland proteins. The assay was designed to assess B. burgdorferi s.s. migration upward against gravity through a transwell polycarbonate membrane overlaid with 6% gelatin. Borreliae that channeled into the upper transwell chamber in response to test proteins were enumerated by flow cytometry. The transwell assay measured chemoattractant activity for B. burgdorferi s.s. from salivary gland extract (SGE) harvested from nymphal ticks during bloodmeal engorgement on mice 42 h post-attachment and saliva collected from adult ticks. Additionally, SGE protein fractions separated by size exclusion chromatography demonstrated various levels of chemoattractant activity in the transwell assay. Sialostatin L, and Salp-like proteins 9 and 11 were identified by mass spectrometry in SGE fractions that exhibited elevated activity. Recombinant forms of these proteins were tested in the transwell assay and showed positive chemoattractant properties compared to controls and another tick protein, S15A. These results were reproducible providing evidence that the transwell assay is a useful method for continuing investigations to find tick saliva components instrumental in driving B. burgdorferi s.s. chemotaxis.
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