4.7 Article

Enhanced vascularization and de novo tissue formation in hydrogels made of engineered RGD-tagged spider silk proteins in the arteriovenous loop model

期刊

BIOFABRICATION
卷 13, 期 4, 页码 -

出版社

IOP PUBLISHING LTD
DOI: 10.1088/1758-5090/ac0d9b

关键词

engineered recombinant spider silk; biofunctionalization; arteriovenous loop; angiogenesis; tissue formation

资金

  1. Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) [326998133-TRR 225]
  2. DFG [SCHE 603/24-1]

向作者/读者索取更多资源

Surgically induced angiogenesis using arteriovenous loops (AVL) is an effective method to improve vascularization of nano-fibrillary recombinant eADF4(C16) hydrogels. Additionally, incorporating RGD-tagged eADF4(C16) into hydrogels can enhance stability and vascularization, potentially optimizing silk-based biomaterials for tissue engineering applications.
Due to its low immunogenic potential and the possibility to fine-tune their properties, materials made of recombinant engineered spider silks are promising candidates for tissue engineering applications. However, vascularization of silk-based scaffolds is one critical step for the generation of bioartificial tissues and consequently for clinical application. To circumvent insufficient vascularization, the surgically induced angiogenesis by means of arteriovenous loops (AVL) represents a highly effective methodology. Here, previously established hydrogels consisting of nano-fibrillary recombinant eADF4(C16) were transferred into Teflon isolation chambers and vascularized in the rat AVL model over 4 weeks. To improve vascularization, also RGD-tagged eADF4(C16) hydrogels were implanted in the AVL model over 2 and 4 weeks. Thereafter, the specimen were explanted and analyzed using histology and microcomputed tomography. We were able to confirm biocompatibility and tissue formation over time. Functionalizing eADF4(C16) with RGD-motifs improved hydrogel stability and enhanced vascularization even outperforming other hydrogels, such as fibrin. This study demonstrates that the scaffold ultrastructure as well as biofunctionalization with RGD-motifs are powerful tools to optimize silk-based biomaterials for tissue engineering applications.

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