期刊
VIRUSES-BASEL
卷 13, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/v13091728
关键词
bocavirus; respiratory tract; prevalence; Croatia; phylogeny; recombination
类别
资金
- Croatian Science Foundation [IP-2016-06-7556, KK.01.1.1.01.0006]
- European Regional Development Fund
The study investigated the prevalence of HBoV genotypes in pediatric patients with respiratory tract infections in Croatia, revealing that 7.6% of children were infected with HBoV, primarily belonging to the HBoV1 genotype. Co-infection was observed in 82.2% of the samples.
Human bocavirus (HBoV) 1 is considered an important respiratory pathogen, while the role of HBoV2-4 in clinical disease remains somewhat controversial. Since, they are characterized by a rapid evolution, worldwide surveillance of HBoVs' genetics is necessary. This study explored the prevalence of HBoV genotypes in pediatric patients with respiratory tract infection in Croatia and studied their phylogeny. Using multiplex PCR for 15 respiratory viruses, we investigated 957 respiratory samples of children up to 18 years of age with respiratory tract infection obtained from May 2017 to March 2021 at two different hospitals in Croatia. Amplification of HBoV near-complete genome or three overlapping fragments was performed, sequenced, and their phylogenetic inferences constructed. HBoV was detected in 7.6% children with a median age of 1.36 years. Co-infection was observed in 82.2% samples. Sequencing was successfully performed on 29 HBoV positive samples, and all belonged to HBoV1. Croatian HBoV1 sequences are closely related to strains isolated worldwide, and no phylogenetic grouping based on mono- or co-infection cases or year of isolation was observed. Calculated rates of evolution for HBoV1 were 10(-4) and 10(-5) substitutions per site and year. Recombination was not detected among sequences from this study.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据