期刊
VIRUSES-BASEL
卷 13, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/v13071332
关键词
Pseudomonas aeruginosa; biofilm; phage-resistant mutants; virulence; heterogeneity
类别
资金
- National Science Centre, Poland [2015/18/M/NZ6/00413]
This study evaluates the biology and phenotype changes in Pseudomonas aeruginosa PAO1 phage-resistant clones that emerge in phage-treated biofilms, characterizing them for phage-typing patterns, antibiotic resistance, biofilm formation, pathogenicity, and interactions with the innate immune system. Despite the similar phage resistance patterns selected by different phages targeting a particular receptor, there is a dramatic increase in population heterogeneity as a result. The findings also suggest that phage-resistant bacteria are more susceptible to antibiotics and clearance mechanisms by the immune system, supporting the application of phages in therapy.
Bacterial surface structures of a proteinic nature and glycoconjugates contribute to biofilm formation and provide shields to host defense mechanisms (e.g., the complement system and phagocytosis). A loss or alteration of these molecules, leading to phage resistance, could result in fewer virulent bacteria. In this study, we evaluate the biology and phenotype changes in Pseudomonas aeruginosa PAO1 phage-resistant clones, which emerge in phage-treated biofilms. We characterize these clones for phage-typing patterns, antibiotic resistance, biofilm formation, pathogenicity, and interactions with the innate immune system. Another important question that we address is whether phage-resistant mutants are also generated incidentally, despite the phage treatment-selective pressure, as the natural adaptation of the living biofilm population. It is found that the application of different phages targeting a particular receptor selects similar phage resistance patterns. Nevertheless, this results in a dramatic increase in the population heterogeneity, giving over a dozen phage-typing patterns, compared to one of the untreated PAO1 sessile forms. We also confirm the hypothesis that phage-resistant bacteria are more susceptible to antibiotics and host-clearance mechanisms by the immune system. These findings support phage application in therapy, although the overall statement that phage treatment selects the less virulent bacterial population should be further verified using a bigger collection of clinical strains.
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