期刊
VIRUSES-BASEL
卷 13, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/v13061042
关键词
metatranscriptomics; virome; codivergence; vertebrate-associated DNA virus
类别
资金
- Guangdong Province Pearl River Talent Plan Innovation and Entrepreneurship Team Project [2019ZT08Y464]
A previous study identified a wide viral diversity of RNA viruses in lower vertebrate hosts, but the diversity of DNA and retro-transcribing viruses remained uncharacterized. This study utilized metatranscriptomics sequencing to reveal complete genome sets of 18 vertebrate-associated DNA and retro-transcribing viruses in cartilaginous fishes, ray-finned fishes, and amphibians, shedding light on the evolutionary relationships within these virus groups.
In a previous study, a metatranscriptomics survey of RNA viruses in several important lower vertebrate host groups revealed huge viral diversity, transforming the understanding of the evolution of vertebrate-associated RNA virus groups. However, the diversity of the DNA and retro-transcribing viruses in these host groups was left uncharacterized. Given that RNA sequencing is capable of revealing viruses undergoing active transcription and replication, we collected previously generated datasets associated with lower vertebrate hosts, and searched them for DNA and retro-transcribing viruses. Our results revealed the complete genome, or core gene sets, of 18 vertebrate-associated DNA and retro-transcribing viruses in cartilaginous fishes, ray-finned fishes, and amphibians, many of which had high abundance levels, and some of which showed systemic infections in multiple organs, suggesting active transcription or acute infection within the host. Furthermore, these new findings recharacterized the evolutionary history in the families Hepadnaviridae, Papillomaviridae, and Alloherpesviridae, confirming long-term virus-host codivergence relationships for these virus groups. Collectively, our results revealed reliable and sufficient information within metatranscriptomics sequencing to characterize not only RNA viruses, but also DNA and retro-transcribing viruses, and therefore established a key methodology that will help us to understand the composition and evolution of the total infectome within a diverse range of vertebrate hosts.
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