期刊
VIRUSES-BASEL
卷 13, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/v13081606
关键词
Newcastle disease virus; thermostability; HA protein; transmembrane domain; recombinant virus
类别
资金
- National Key Research and Development Project of China [2016YFD0501604, 2016YFD0501609]
- China Agriculture Research System of MOF and MARA [CARS-40]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
This study developed recombinant NDVs expressing HA using a thermostable NDV vector, which maintained their thermostable and lentogenic nature. Immunization data showed that rAHR09-HA and rAHR09-HAF induced a higher HI antibody titer against H9N2 AIV and NDV, and reduced virus shedding after challenge. These results suggest that rAHR09-HA and rAHR09-HAF may be vaccine candidates against H9N2 AIV.
H9N2 avian influenza virus (AIV) has become endemic in many countries, causing great economic losses when co-infected with other pathogens. So far, several live vaccines based on Newcastle disease virus (NDV) vectors expressing influenza hemagglutinin (HA) have been developed. However, the thermostable recombinant NDV is rarely reported. In this study, using a thermostable NDV rAHR09 strain as the vector, three recombinant NDVs expressing native HA, chimeric HA ectodomain with transmembrane domain/C-terminal cytoplasmic tail domain from fusion protein of NDV, and HA ectodomain were generated, designated rAHR09-HA, rAHR09-HAF, and rAHR09-HAE. The MDT value of three recombinant NDVs was above 120 h, their ICPI value was about 0.03, and the recombinant NDVs were still infectious when treated for 100 min under 56 degrees C, which demonstrated that the recombinant NDVs kept the lentogenic and thermostable nature of rAHR09. The immunization data showed that rAHR09-HA and rAHR09-HAF induced a higher HI antibody titer against H9N2 AIV and NDV. After being challenged with H9N2 AIV, the rAHR09-HA and rAHR09-HAF could significantly reduce the virus shedding in cloacal and tracheal swab samples. Our results suggest that rAHR09-HA and rAHR09-HAF might be vaccine candidates against H9N2 AIV.
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