期刊
VIRUSES-BASEL
卷 13, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/v13061036
关键词
Senecavirus A; proteomics; differentially expressed protein; enrichment analysis; metabolism; pathway
类别
资金
- Innovation Fund - China Animal Health and Epidemiology Center
This study used proteomics and metabolomics methods to investigate the impact of SVA infection on the proteomic profile of BSR-T7/5 cells. It was found that 361 differentially expressed proteins were identified in SVA-infected cells at 12 hours post-inoculation, with 305 upregulated and 56 downregulated proteins. GO and KEGG enrichment analyses indicated that cellular metabolisms were mainly affected in the early stage of SVA infection.
Senecavirus A (SVA), also known as Seneca Valley virus, belongs to the genus Senecavirus in the family Picornaviridae. SVA can cause vesicular disease and epidemic transient neonatal losses in pigs. This virus efficiently propagates in some non-pig-derived cells, like the baby hamster kidney (BHK) cell line and its derivate (BSR-T7/5). Conventionally, a few proteins or only one protein is selected for exploiting a given mechanism concerning cellular regulation after SVA infection in vitro. Proteomics plays a vital role in the analysis of protein profiling, protein-protein interactions, and protein-directed metabolisms, among others. Tandem mass tag-labeled liquid chromatography-tandem mass spectrometry combined with the parallel reaction monitoring technique is increasingly used for proteomic research. In this study, this combined method was used to uncover separately proteomic profiles of SVA- and non-infected BSR-T7/5 cells. Furthermore, both proteomic profiles were compared with each other. The proteomic profiling showed that a total of 361 differentially expressed proteins were identified, out of which, 305 and 56 were upregulated and downregulated in SVA-infected cells at 12 h post-inoculation, respectively. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analyses showed that cellular metabolisms were affected mainly in SVA-inoculated cells at an early stage of infection. Therefore, an integrated metabolic atlas remains to be explored via metabolomic methods.
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