期刊
VIRUSES-BASEL
卷 13, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/v13060975
关键词
human norovirus; human rotavirus; human astrovirus; gastroenteritis; immortalized cell lines; human enteroids
类别
资金
- NIH [R01 AI127552, R01 AI139314, R01 AI141478]
- Children's Discovery Institute of Washington University
- St. Louis Children's Hospital Interdisciplinary Research Initiative grant [MI-II-2019-790]
- Pew Biomedical Scholars Program
- Mathers Foundation
This article discusses the methodological advancements and tools available for studying human enteric RNA viruses, including in vitro and in vivo experiments. It provides insights into many features of viral pathogenesis and unclear aspects, highlighting key areas and opportunities that require further research.
Every year, millions of children are infected with viruses that target the gastrointestinal tract, causing acute gastroenteritis and diarrheal illness. Indeed, approximately 700 million episodes of diarrhea occur in children under five annually, with RNA viruses norovirus, rotavirus, and astrovirus serving as major causative pathogens. Numerous methodological advancements in recent years, including the establishment of novel cultivation systems using enteroids as well as the development of murine and other animal models of infection, have helped provide insight into many features of viral pathogenesis. However, many aspects of enteric viral infections remain elusive, demanding further study. Here, we describe the different in vitro and in vivo tools available to explore different pathophysiological attributes of human enteric RNA viruses, highlighting their advantages and limitations depending upon the question being explored. In addition, we discuss key areas and opportunities that would benefit from further methodological progress.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据