期刊
VOX SANGUINIS
卷 117, 期 3, 页码 399-407出版社
WILEY
DOI: 10.1111/vox.13184
关键词
column agglutination technology; cost; failure mode and effect analysis; isoagglutinin titre; tube test; turnaround time; VISION Max
类别
资金
- Konkuk University
The study compared the measurement of blood type antibodies using automated column agglutination technology and tube test, finding high agreement and correlation between the two methods for most blood types. Modified CAT showed superior performance in terms of risk analysis, turnaround time, and cost.
Background and Objectives VISION Max (Ortho Clinical Diagnostics, Raritan, NJ) measures anti-A/B isoagglutinin titres using automated column agglutination technology (CAT). We compared tube test (TT) and CAT of VISION Max comprehensively, including failure mode and effect analysis (FMEA), turnaround time (TAT) and cost, and suggested modified CAT (MCAT). Materials and Methods For 100 samples (each 25 for blood type A, B and O with anti-A and anti-B), anti-A/B isoagglutinin titres were measured by TT and CAT (1:2-1:1024 dilution), as well as by MCAT (with agglutination at 1:32 dilution, then perform additional testing from 1:64 to 1:1024). We assessed the agreement and correlation between TT and CAT and compared FMEA (risk priority number [RPN] score), TAT (h:min:sec) and cost (US dollar, US $) among TT, CAT and MCAT. Results TT and CAT showed overall substantial agreement (k = 0.73) and high correlation (rho >= 0.75) except blood type O with anti-A (rho = 0.68). Compared with TT, CAT showed lower RPN scores in FMEA and similar TAT and cost (FMEA, 33,700 vs. 184,300; TAT, 15:23:00 vs. 14:26:40; cost, 1377.4 vs. 1312.4, respectively). Regarding FMEA, TAT and cost, MCAT was superior to CAT or TT (43,810; 13:28:00; 899.2, respectively). Conclusion This is the first multidimensional analysis on VISION Max CAT for measuring anti-A/B isoagglutinin titres. The results of anti-A/B isoagglutinin titres by CAT were comparable with those of TT. MCAT would be a safe, time-saving and cost-effective alternative to TT and CAT in high-volume blood bank laboratories.
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