期刊
VIRUS RESEARCH
卷 302, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.virusres.2021.198497
关键词
Porcine deltacoronavirus; N protein; Epitopes
类别
资金
- Key Scientific Research Projects of Heilongjiang Province Farms & Land Reclamation Administration [201914]
- Heilongjiang Bayi Agricultural University [TDJH201804]
The study identified a novel linear B cell epitope of the PDCoV N protein, termed EP-9G1, recognized by the 9G1 monoclonal antibody. The epitope was found to be highly conserved among different coronavirus genera, providing potential for developing diagnostic reagents for PDCoV.
Porcine deltacoronavirus (PDCoV) is an emerging coronavirus that causes vomiting, diarrhea, dehydration, and even death of piglets, resulting in significant losses to the pig industry worldwide. However, the epitopes of PDCoV remain largely unknown. In this study, a monoclonal antibody (mAb) against the PDCoV nucleocapsid (N) protein, termed 9G1, was prepared using the lymphocyte hybridoma technique, and was identified as a type IgG1 with a kappa light chain and reacted with the native N protein of PDCoV. Furthermore, the epitope recognized by the 9G1 mAb was subjected to western blot and an ELISA using truncated recombinant proteins and synthetic polypeptides of the PDCoV N protein. The results indicate that 9G1 mAb recognized the epitope, G59TPIPPSYAFYY70 (EP-9G1), a novel linear B cell epitope of the PDCoV N protein. A comparison analysis revealed that the EP-9G1 epitope was highly conserved among PDCoV strains, in which four residues (G59-F68YY70) were observed among different coronavirus genera. These data demonstrate that the EP-9G1 epitope identified in this study provides some basic information for further characterization of the antigenic structure of the PDCoV N protein and has potential use for developing diagnostic reagents for PDCoV.
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