期刊
VIRUS RESEARCH
卷 298, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.virusres.2021.198406
关键词
Bacteriophage propagation; Phage therapy; Staphylococcus aureus; Process development; Design of experiments; Definitive screening design
类别
资金
- Japan Society for the Promotion of Science KAKENHI [JP18K07127]
- Azabu University, Japan
The study found that the propagation processes of phages need to be tailored according to the type of phage, and careful consideration should be given to the choice of methodologies for measuring phage concentration.
To introduce phage therapy against multidrug-resistant Staphylococcus aureus in Western medicine, the establishment of phage manufacturing, particularly phage propagation, is indispensable. For the propagation of S. aureus phages, knowledge of the effects of phage types, process parameters, and analytical methodologies should be investigated. In this study, S. aureus phage propagations were studied in a flask with a new class of design of experiments, definitive screening design, using S. aureus phages S13' and S25-3 in different taxonomies. Four process parameters, namely, multiplicity of infection, bacterial density at infection, time of harvest, and temperature, were evaluated with the regression models based on the phage concentration data measured using plaque assay and quantitative polymerase chain reaction. As a result, phage propagations measured using plaque assay and quantitative polymerase chain reaction were overall similar to each other in the case of phage S13', while they differed in the case of phage S25-3. These results suggest that the propagation processes need to be developed according to phage type, and the choice of methodologies for phage concentration measurements should be carefully considered.
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