期刊
VIROLOGY
卷 560, 期 -, 页码 66-75出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2021.05.007
关键词
Anti-HBV; Hepatitis B virus (HBV); 3,5,6,7,3 ',4 '-hexamethoxyflavone; Hepatitis B surface Antigen (HBsAg); Hepatocyte nuclear factor 3 alpha (HNF3 alpha); Therapeutic drug
类别
资金
- National Natural Science Foundation of China [81861168035, 81922011, 81871656, 81702088]
- National Science and Technology Major Project [2017ZX10202203]
- Creative Research Group of CQ University [CXQT19016]
- Chongqing Natural Science Foundation [cstc2018jcyjAX0114]
3,5,6,7,3',4'-Hexamethoxyflavone showed anti-HBV effects by reducing HBV RNA, HBV DNA, and HBsAg levels, as well as inhibiting HBV promoter activity and altering the RNA/cccDNA ratio. The compound has the potential to be a therapeutic drug for hepatitis B virus.
Current treatment methods for hepatitis B are mainly antiviral drug therapy and immunotherapy, in which antiviral drugs are mainly nucleoside analogue and interferon. They can significantly reduce the viral load but rarely achieve hepatitis B surface antigen (HBsAg) loss. 3,5,6,7,3',4'-Hexamethoxyflavone was screened out from a small molecule compound library for its lower cytotoxic effect and greater HBsAg inhibition activity. Meanwhile, we further performed experiments in HepG2.2.15, HepG2-NTCP cells, PHHs and HBV transgenic mouse model to evaluate the anti-HBV effects of 3,5,6,7,3',4'-Hexamethoxyflavone. Our study found that 3,5,6,7,3',4'-Hexamethoxyflavone can significantly reduce the level of HBV RNAs, HBV DNA and HBsAg, in addition, the activity of four HBV promoters and the ratio of total RNAs/cccDNA and 3.5 kb RNA/cccDNA were decreased by 3,5,6,7,3',4'-Hexamethoxyflavone. Mechanistically, we found HNF3 alpha plays important roles in Hex mediated HBV transcription inhibition. Our study indicated Hex was a potential anti-HBV therapeutic drug.
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