4.7 Article

Streptococcus equi-derived extracellular vesicles as a vaccine candidate against Streptococcus equi infection

期刊

VETERINARY MICROBIOLOGY
卷 259, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.vetmic.2021.109165

关键词

Streptococcus equi; Extracellular vesicle; Proteomics-based reverse vaccinology; Potential vaccine candidate; Vaccination; Immunoprecipitation-MS

资金

  1. Korea Basic Science Institute research program [C180310]
  2. National Council of Science & Technology (NST) grant from the Korean government (MSIP) [CRC-16-01-KRICT]
  3. National Research Council of Science & Technology (NST), Republic of Korea [C180310] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)

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The study demonstrates the potential of Streptococcus equi-derived extracellular vesicles (EVs) as a vaccine candidate against S. equi infection, showing improved survival rates in mice and containing various immunogenic antigens within the EV proteome.
Streptococcus equi subspecies equi is a pathogenic bacterium that causes strangles, a highly contagious respiratory infection in horses and other equines. The limitations of current vaccines against S. equi infection warrants the development of an affordable, safe, and effective vaccine. Because gram-positive extracellular vesicles (EVs) transport various immunogenic antigens, they are attractive vaccine candidates. Here, we purified the EVs of S. equi ATCC 39506 and evaluated them as a vaccine candidate against S. equi infection in mice. As an initial step, comparative proteomic analysis was performed to characterize the functional features of the EVs. Reverse vaccinology and knowledge-based annotations were then used to screen potential vaccine candidates (PVCs) for S. equi ATCC 39506. Finally, 32 PVCs were found to be enriched in the EV fraction, suggesting the usefulness of this fraction as a vaccine. Importantly, a significantly higher survival rate after S. equi infection was detected in mice immunized with S. equi-derived EVs via the intraperitoneal route than in mice immunized with heat-killed bacteria. Of note, immunoprecipitation-mass spectrometry results validated various immunogenic antigens within the EV proteome. In conclusion, our results suggest that S. equi-derived EVs can serve as a vaccine candidate against S. equi infection.

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