期刊
VETERINARY MICROBIOLOGY
卷 258, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.vetmic.2021.109126
关键词
M. bovis; Caspase-1; IL-1 beta; IFN-beta; cGAS
资金
- National Natural Science Foundation of China [31873005]
- China Agriculture Research System [CARS36]
- National Key Research and Development Program [2017YFD0500901]
- MOSTRCUK international cooperation project [2013DFG32500]
- Highend Foreign Experts Recruitment Program [GDW20151100036, GDW20161100071]
The inflammasome protects hosts by negatively regulating harmful cytokines during M. bovis infection, as shown in the research finding that caspase-1 activation reduces IFN-beta production by dampening cGAS-TBK1-IRF3 signaling pathway.
Mycobacterium bovis (M. bovis) infection triggers cytokine production via pattern recognition receptors. These cytokines include type I interferons (IFNs) and interleukin-1 beta (IL-1 beta). Excessive type I IFN levels impair host resistance to M. bovis infection. Therefore, strict control of type I IFN production is helpful to reduce pathological damage and bacterial burden. Here, we found that a deficiency in caspase-1, which is the critical component of the inflammasome responsible for IL-1 beta production, resulted in increased IFN-beta production upon M. bovis infection. Subsequent experiments demonstrated that caspase-1 activation reduced cyclic GMP-AMP synthase (cGAS) expression, thereby inhibiting downstream TANK-binding kinase 1 (TBK1)-interferon regulatory factor 3 (IRF3) signaling and ultimately reducing IFN production. A deficiency in caspase-1 activation enhanced the bacterial burden during M. bovis infection in vitro and in vivo and aggravated pathological lesion formation. Thus, caspase-1 activation reduced IFN-beta production upon M. bovis infection by dampening cGAS-TBK1-IRF3 signaling, suggesting that the inflammasome protects hosts by negatively regulating harmful cytokines.
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