Opinion Understanding and interpreting mosquito blood feeding studies: the case of Aedes albopictus

Blood feeding is a fundamental mosquito behavior with consequences for pathogen transmission and control. Feeding behavior can be studied through two lenses - patterns and preference. Feeding patterns are assessed via blood meal analyses, reflecting mosquito-host associations influenced by environmental and biological parameters. Bias can profoundly impact results, and we provide recommendations for mitigating these effects. We also outline design choices for host preference research, which can take many forms, and highlight their respective (dis)advantages for preference measurement. Finally, Aedes albopictus serves as a case study for how to apply these lessons to interpret data and understand feeding biology. We illustrate how assumptions and incomplete evidence can lead to inconsistent interpretations by reviewing Ae. albopictus feeding studies alongside prevalent narratives about perceived behavior.

Automated summary

This article explores the importance of studying mosquito blood feeding behavior through patterns and preferences. Biases can significantly impact results, and recommendations are provided to mitigate these effects. Additionally, design choices for host preference research are crucial for accurate measurement of preferences.