期刊
TRANSLATIONAL ONCOLOGY
卷 14, 期 8, 页码 -出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.tranon.2021.101125
关键词
P-selectin glycoprotein ligand 1; Lymphoma; T cell; Tumorigenesis; Dissemination
类别
资金
- Fundacao para a Ciencia e a Tecnologia (Portugal)
- European Social Fund
- European Regional Development Fund [PTDC/SAU-OBD/103336/2008, PTDC/MED-ONC/32592/2017, UID/BIM/04773/2013, NORTE-01-0145-FEDER-000029, POCI-01-0145-FEDER-007274, IF/00056/2012, SFRH/BD/147979/2019]
- Gilead Sciences Portugal [PGG/038/2017]
- i3S Scientific Platform Histology and Electron Microscopy [PPBI-POCI-01-0145-FEDER-022122]
- Fundação para a Ciência e a Tecnologia [PTDC/SAU-OBD/103336/2008, UID/BIM/04773/2013, PTDC/MED-ONC/32592/2017, SFRH/BD/147979/2019] Funding Source: FCT
PSGL-1, a membrane-bound glycoprotein expressed in lymphoid and myeloid cells, plays a role in T cell trafficking and homing, and has been linked to lymphoid malignancies. The expression of PSGL-1 promotes T cell lymphoma development and dissemination to various organs.
P-selectin glycoprotein ligand-1 (PSGL-1) is a membrane-bound glycoprotein expressed in lymphoid and myeloid cells. It is a ligand of P-, E-and L-selectin and is involved in T cell trafficking and homing to lymphoid tissues, among other functions. PSGL-1 expression has been implicated in different lymphoid malignancies, so here we aimed to evaluate the involvement of PSGL-1 in T cell lymphomagenesis and dissemination. PSGL-1 was highly expressed at the surface of human and mouse T cell leukemia and lymphoma cell lines. To assess its impact on T cell malignancies, we stably expressed human PSGL-1 (hPSGL-1) in a mouse thymic lymphoma cell line, which expresses low levels of endogenous PSGL-1 at the cell surface. hPSGL-1-expressing lymphoma cells developed subcutaneous tumors in athymic nude mice recipients faster than control empty vector or parental cells. Moreover, the kidneys, lungs and liver of tumor-bearing mice were infiltrated by hPSGL-1-expressing malignant T cells. To evaluate the role of PSGL-1 in lymphoma cell dissemination, we injected intravenously control and hPSGL1-expressing lymphoma cells in athymic mice. Strikingly, PSGL-1 expression facilitated disease infiltration of the kidneys, as determined by histological analysis and anti-CD3 immunohistochemistry. Together, these results indicate that PSGL-1 expression promotes T cell lymphoma development and dissemination to different organs.
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