Liquid chromatography using ≤5 μm open tubular columns

Significant progresses have been made recently in liquid chromatography (LC) using <= 5-mu m-i.d. open tubular (OT) columns. Peak capacities of 2000+ within 3 h, efficiencies of 10+ million plates/m and millisecond separations have all been obtained. Since all these magnificent progresses are achieved in the last 3-4 years and no one has reviewed these achievements, it is important to write a short review on this topic so that scientists can adopt it in their research and applications. In this article we first summarize the most recent progresses of LC separations using <= 5-mu m-i.d. OT columns. We then review the procedures of how to prepare these columns and share our experiences on how to choose appro-priate experimental components to construct apparatus and select appropriate experimental parameters to carry out these separations. We also discuss the pros and cons and provide a future perspective of this technique at the end of this article. (c) 2021 Elsevier B.V. All rights reserved.

Automated summary

Significant progresses have been made recently in liquid chromatography using <= 5-mu m-i.d. open tubular columns, enabling peak capacities of 2000+, efficiencies of 10+ million plates/m, and millisecond separations. This review article summarizes the latest advancements, procedures for preparing columns, selecting experimental components, and discusses the future prospects of this technique.