期刊
TOXICON
卷 198, 期 -, 页码 64-72出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.toxicon.2021.04.016
关键词
Hesperetin; Serine proteases; Inhibitors; Viperidae family
资金
- CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
- FAPESP (Sao Paulo Research Foundation) [2016/21682-0]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [16/21682-0] Funding Source: FAPESP
90% of snakebite envenomation in Brazil is caused by Bothrops snakes from the Viperidae family, with a serine protease from Bothrops jararaca chosen for study. The study found that hesperetin can act as a mixed inhibitor for the serine protease from Bothrops jararaca snake venom, potentially helping to reverse on-site blood clotting problems after snakebite accidents. Further research is needed to determine the full impact of hesperetin in this context.
The majority (90%) of the snakebite envenomation in Brazil accounts for Bothrops from the Viperidae family. Some snake venom serine proteases provoke blood coagulation in ophidian accident victims because of their fibrinolytic activity, one of those proteases from Bothrops jararaca (B. jararaca) has been chosen for this study. Our objectives were to isolate and characterize the target serine protease; isolate, purify, and characterize the orange bagasse flavone (hesperetin, Hst), and investigate the interactions between the targets, enzyme, and hesperetin. The purified serine protease was named BjSP24 because of its molecular mass and proteolytic activity. BjSP24 was folded and characterized using circular dichroism and showed low alpha-helix contents (7.7%). BjSP24 exhibited sequence similarity to other known snake venom serine proteases as measured in the enzyme tryptic peptides' LC-MS/MS run. Hesperetin was obtained within the expected yield and with the predominance of 2S isomer (82%). It acted as a mixed inhibitor for the serine protease (SVSP) from Bothrops jararaca snake venom observed in three different in vitro experiments, fluorescence, kinetics, and SSTD-NMR. It is still to determine if hesperetin might aid-in reverting the on site blood clotting problems just after snakebite accidents.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据