期刊
TOXICOLOGY LETTERS
卷 343, 期 -, 页码 44-55出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2021.02.015
关键词
Nicotine Hepatic fibrosis; Wnt pathway; Methylation; Intergenerational transmission
类别
资金
- Shanghai Municipal Natural Science Foundation [20ZR1427400]
Paternal nicotine exposure can induce hepatic fibrosis in offspring by down-regulating mmu-miR-15b expression via hyper-methylation, activating the Wnt pathway, leading to increased activation and proliferation of hepatic stellate cells. Additionally, nicotine can promote the activation of the TGF-beta pathway, cooperatively enhancing the transcription of extracellular matrix genes, ultimately contributing to liver fibrosis in offspring.
Paternal nicotine exposure can alter phenotypes in future generations. The aim of this study is to explore whether paternal nicotine exposure affects the hepatic repair to chronic injury which leads to hepatic fibrosis in offspring. Our results demonstrate that nicotine down regulates mmu-miR-15b expression via the hyper-methylation on its CpG island shore region in the spermatozoa. This epigenetic modification imprinted in the liver of the offspring. The decreased mmu-miR-15b promotes the expression of Wnt4 and activates the Wnt pathway in the offspring mice liver. The activation of the Wnt pathway improves the activation and proliferation of hepatic stellate cells (HSCs) leading to liver fibrosis. Moreover, the Wnt pathway promotes the activation of the TGF-beta pathway and the two pathways cooperate to promote the transcription of extracellular matrix (ECM) genes. In conclusion, this study found that nicotine promotes hepatic fibrosis in the offspring via the activation of Wnt pathway by imprinting the hyper-methylation of mmu-miR-15b. (C) 2021 Elsevier B.V. All rights reserved.
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