A Tramadol Drug Electrochemical Sensor Amplified by Biosynthesized Au Nanoparticle Using Mentha aquatic Extract and Ionic Liquid

In this research, the Au nanoparticle was synthesized using a biosynthesized strategy by Mentha aquatic extract and characterized by Uv-vis spectroscopic method. The synthesized Au/NPs were used as a conductive mediator for the modification of the tramadol electrochemical sensor. The modified paste electrode with Au/NPs and 1-butyl-3-methylimidazolium tetrachloroborate (BMTCB) showed high catalytic activity for the determination of tramadol in aqueous solution. When compared to the bare electrode, the Au/NPs/BMTCB/CPE increased tramadol oxidation current by 8.8 times and lowered drug oxidation potential by 20 mV. The diffusion coefficient (D) similar to 2.55 x 10(- 5) cm(2)/s was calculated for electro-oxidation of tramadol at surface of Au/NPs/BMTCB/CPE. With a detection limit of 6.0 nM, the Au/NPs/BMTCB/CPE revealed a linear relationship between tramadol oxidation current and concentration in the range of 0.01-400.0 M.

Automated summary

In this study, gold nanoparticles were successfully synthesized using a biosynthetic strategy and used to modify an electrochemical sensor with high catalytic activity for detecting tramadol in aqueous solution. The modified sensor showed 8.8 times higher tramadol oxidation current and 20 mV lower drug oxidation potential compared to the bare electrode, with a detection limit of 6.0 nM and a linear relationship between tramadol oxidation current and concentration in the range of 0.01-400.0 M.