期刊
TISSUE & CELL
卷 70, 期 -, 页码 -出版社
CHURCHILL LIVINGSTONE
DOI: 10.1016/j.tice.2021.101503
关键词
Pleura; Friction; MUC1; Fibrine gel; Mesothelial cells; Neuraminidase; Dexamethasone
资金
- Ministero dell'Istruzione, dell'Universit`a e della Ricerca, Italy
The study investigated the role of sialomucin in friction reduction by studying the sliding friction of pleural mesothelial cells under different experimental conditions. Results showed that sialomucin may play a role in reducing the friction of these cells.
To elucidate the role of sialomucin in friction reduction, we investigated the sliding friction of pleural mesothelial cells monolayers cultured on fibrine gel. These measurements were performed on normal (4/4 RM-4) and on tumor (CARM-L1 TG3) cell lines. The effect of treatment with neuraminidase, which removes sialic acid from sialomucin, and of dexamethasone, which has shown to increase sialomucin expression, were also assessed. Furthermore, the expression of the main form of cell-surface-associated mucin (MUC1) present in the mesothelium, was assessed by western blot and immunofluorescence, under different experimental conditions. Expression of MUC1 was not significantly different in the two cell lines. Moreover, dexamethasone did not increase the expression of MUC1. Coefficient of kinetic friction (mu) was significantly higher in tumor cells than in normal cells. Neuraminidase increased mu in both cell lines. These results suggest that sialomucin may play a role in reducing the friction of pleural mesothelial cells.
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