期刊
TISSUE & CELL
卷 71, 期 -, 页码 -出版社
CHURCHILL LIVINGSTONE
DOI: 10.1016/j.tice.2021.101576
关键词
Breast cancer; Exosomes; Glycolysis; GLUT1; HK2; PD1
资金
- Ahvaz Jundishapur University of Medical Sciences [CRC-9910]
Tumor cells modulate immune responses by secreting exosomes, which affect the metabolism of immune cells via the PD1-GLUT1-HK2 metabolic axis. Exosomes from breast cancer patients increase the expression of key genes in the glycolysis pathway in PBMCs.
Tumor cells modulate immune responses by secreting exosomes. Tumor exosomes can affect the metabolism of immune cells and increase immune inhibitory molecules such as programmed cell death protein 1 (PD-1). PD-1 inhibits the glycolysis pathway in immune cells. We investigated the role of tumor exosomes in how metabolic changes occur through the PD1-GLUT1-HK2 metabolic axisin peripheral blood mononuclear cells (PBMCs). The MDA-MB-231 cell line was cultured, serum samples from breast cancer patients were collected, and exosomes purified from serum samples and the MDA-MB-231 cell line. PBMCs were treated with purified exosomes for 72 h and, the expression of PD1-GLUT1-HK2 genes was measured by real-time PCR. Our study results showed relative expression of the HK2 gene in both groups treated with MDA-MB-231 cell line exosomes and serum exosomes of breast cancer patients was significantly increased compared to the control group (p < 0.0001). Also, the relative expression of the PD1 gene and GLUT1 gene showed a significant increase compared to the control group only in the group treated with MDA-MB-231 cell line exosomes (p < 0.0001). Therefore, Breast cancer exosomes increased the expression of key genes in the glycolysis pathway, increasing the glycolysis pathway in PBMCs. Increased expression of PD-1 could not prevent the expression of critical genes in the glycolysis pathway as in previous studies.
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