期刊
THERIOGENOLOGY
卷 168, 期 -, 页码 25-32出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2021.03.026
关键词
Spermidine; Mouse; Ovary; Oxidative stress; Granulosa cell; Apoptosis
资金
- National Natural Science Foundation of China [31872358, 31702116]
- Scientific Research Fund of Sichuan Provincial Education Department [18ZB0469]
This study demonstrates for the first time that supraphysiological doses of spermidine cause ovarian oxidative stress and induce granulosa cell apoptosis, potentially through the P53 and/or BCL2-CASPASEs pathway.
Given that spermidine is associated with aging-related diseases and it is a potential target for delaying aging, functional studies on supraphysiological levels of spermidine are required. Our previous studies showed that the granulosa layer arranged irregular and the follicular oocytes were shrunk in female mice injected intraperitoneally with spermidine at 150 mg/kg (Body weight) after 24 h. It indicated that supraphysiological levels of spermidine induced ovarian damage in female mice. The objective of this study was to investigate the effect of acute administration of supraphysiological spermidine on the ovary and granulosa cells in mice. The results showed that treatment with spermidine at 150 mg/kg (intraperitoneal) significantly increased the levels of both H2O2 and malondialdehyde and reduced total antioxidant capacity and the activities of catalase and superoxide dismutase in mouse ovaries. The contents of putrescine and spermine increased significantly in the ovaries of mice treated with spermidine. Treatment with spermidine at 150 mg/kg increased the apoptotic rate and reactive oxygen species levels of granulosa cells in mouse ovaries. Furthermore, the protein expression of P53, CASPASE 8 (Cleaved/Pro), CASPASE 9 (Cleaved/Pro) and CASPASE 3 (Cleaved/Pro) in granulosa cells of mice treated with spermidine were significantly upregulated, while BCL2 expression was significantly downregulated. In summary, our study demonstrates for the first time that spermidine at supraphysiological doses causes ovarian oxidative stress and induces granulosa cell apoptosis via the P53 and/or BCL2-CASPASEs pathway. (C) 2021 Elsevier Inc. All rights reserved.
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