期刊
THERIOGENOLOGY
卷 171, 期 -, 页码 21-29出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2021.05.004
关键词
Ram spermatozoa; Cryopreservation; Trehalose; iTRAQ; PRM
资金
- National Natural Science Foundation of China [31960663, 31560635]
- National Wool Caprine Industrial Technology System of China [CARS-39]
Trehalose has been shown to enhance post-thaw quality of ram spermatozoa by modifying their protein profile, potentially through antioxidation, involvement in glycolysis, and increasing spermatozoa tolerance to various stresses.
As a magical oligosaccharide, trehalose has been revealed to enhance the post-thaw quality of stock semen. However, information regarding the cryoprotective mechanism of trehalose during cryopreser-vation has not yet been determined. This study was designed to observe the effects of trehalose on the proteome of ram frozen spermatozoa by applying the isobaric tag for relative and absolute quantification (iTRAQ) strategy combined with parallel reaction monitoring (PRM). A total of 1269 proteins were identified. Among them, there were 21 differentially expressed proteins (DEPs), with 9 up-regulated proteins and 11 down-regulated proteins in spermatozoa frozen with trehalose. These DEPs were pri-marily located in nucleus, cytoplasm, and extracellular region. The Gene Ontology (GO) enrichment analysis demonstrated the involvement of the DEPs in signal transduction, ion binding, oxidoreductase activity, response to stress, and catabolic processes. Based on the STRING analysis, tight functional cor-relations were observed between 6-phosphogluconate dehydrogenase, fructose-bisphosphate aldolase A isoform 1, 14-3-3 protein epsilon, tyrosine-protein kinase Fer, and beta-hexosaminidase subunit alpha precursor. Furthermore, 10 DEPs were verified using PRM, confirming the accuracy of the iTRAQ data acquired in this study. In conclusion, trehalose can modify the protein profile of ram spermatozoa during cryopreservation, which may be associated with its cryoprotective effects. Additionally, trehalose may function on frozen spermatozoa through antioxidation, involvement in glycolysis, and increment of spermatozoa tolerance to various stresses. (c) 2021 Elsevier Inc. All rights reserved.
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