期刊
TALANTA
卷 228, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.talanta.2021.122199
关键词
Bi-functional MOF; microRNA; Fluorescence; Ratiometric
资金
- National Natural Science Foundation of China [21775132]
- National Natural Science Foundation of Hunan province [2018JJ2388]
- Hunan Collaborative Innovation Center of Chemical Engineering & Technology with Environmental Benignity and Effective Resource Utilization
- project of innovation team of the ministry of education [IRT_17R90]
In this study, a metal-organic framework with intrinsic fluorescence and fluorescence quenching ability was designed for high-performance miRNA detection, showing good sensitivity and selectivity for detecting miRNA-203. The ratiometric fluorescent platform can eliminate interference caused by the sensing environment, providing accurate detection results in laboratory and biological samples.
Herein, a metal-organic framework (UiO-66-NH2) with two functions (intrinsic fluorescence and fluorescence quenching ability) is designed to establish a ratiometric fluorescent platform for high-performance miRNA detection. The use of a fluorescent organic ligand endows the MOF material with a strong intrinsic fluorescence at 440 nm. In the presence of target miRNA, the fluorescence signal of the FAM is restored with the triple helix molecular beacons bind to the target. Using the I-FAM/I-MOF signal as the output, the prepared ratiometric probe was able to eliminate disturbance caused by the sensing environment. Under the optimal reaction conditions, including buffer pH of 7.4, temperature of 37 degrees C, and response time of 1 h, the best detection results can be obtained. The ratiometric fluorescence probe presented showed good sensitivity and selectivity for detecting miRNA-203 and the limit of detection was 400 pM with a wide linear range from 1 nM to 160 nM. In addition, this method was applied to diluted human serum and cell lysates, and good detection effect was realized.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据