期刊
TALANTA
卷 228, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.talanta.2021.122221
关键词
F; S-BN QD; Ag3PO4 NP@MoS2 nanosheet; ECL sensor; Tumor gene detection
资金
- Natural Science Foundation of Jilin Province [20200201301JC]
A novel Ag3PO4 NPs@MoS2 nanosheet-based electrochemiluminescence sensing system was developed for tumor gene detection, with optimized ECL emitter and catalyst. The biosensor designed in this study can accurately quantify K-ras tumor gene, demonstrating significant potential value in cancer detection.
In this research, a novel Ag3PO4 NPs@MoS2 nanosheet-based electrochemiluminescence (ECL) sensing system was developed to provide an effective method for tumor gene detection. At first, fluorine, sulfur-doped BN quantum dot (F, S-BN QD) were prepared as ECL emitter. Sulfur dopant can provide more reactive sites in the ECL reaction. Fluorine atoms in the QD structure further improved the stability of the crystal. Furthermore, Ag3PO4 NP@MoS2 nanosheets were fabricated via a hydrothermal route as ECL reaction catalyst. On the one hand, Ag3PO4 NP@MoS2 nanosheets promoted the generation of more oxidant of coreactant in the F, S-BN QD/H2O2 coreactant ECL pathway. On the other hand, the excellent conductivity of Ag3PO4 NP@MoS2 nanosheets facilitated the electron transfer and effectively reduce the damage of F, S-BN QD by excessive hot electrons. Finally, the proposed biosensor was designed to accurately quantify K-ras tumor gene from 10 fM to 100 pM with a limit of detection (LOD) of 0.2 fM. The sensing system was used to detect K-ras gene in human colorectal cancer tumor and tumor-adjacent tissues samples with satisfactory results. The amplified ECL sensing strategy with Ag3PO4 NPs@MoS2 nanosheet has significant potential value in the clinical detection.
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