A hydrolytically stable amide polar stationary phase for hydrophilic interaction chromatography

A novel amide functionalized polar stationary phase has been described by grafting acrylamide polymer coating onto hydrolysed poly (glycidyl methacrylate-divinylbenzene) (GMA-DVB) microsphere. The grafting of acrylamide coating is performed via free radical polymerization of acrylamide with pendant double bonds of hydrolysed GMA-DVB microsphere. The obtained stationary phase (G-pAM) possesses favorable hydrophilicity, as proved by strong retention and good separation ability towards several types of model polar analytes (e.g. 71913 plates/m plate count of sucrose), and excellent hydrolytically stability, as indicated by extremely low bleed level (much superior to commercial ones, similar to 23.7-fold-similar to 77.4-fold lower). Negligible baseline drift under gradient elution (water fraction even up to 50%) was observed. It also exhibits good selectivity in the separation of isomers and homologue sugars.

Automated summary

The novel amide functionalized polar stationary phase exhibits favorable hydrophilicity, excellent separation ability, and outstanding hydrolytic stability, with minimal baseline drift under gradient elution and good selectivity in the separation of isomers and homologue sugars.