Photophysics of thiazole orange in deep eutectic solvents

Photophysics and torsional dynamics of thiazole orange (TO) as a function of temperature have been studied in two deep eutectic solvents (DESs) using spectroscopic techniques. Two DESs are used as a solvent namely DES-I (choline chloride + urea, mole ratio 1: 2) and DES-II (N,N diethyl ethanol ammonium chloride + urea, mole ratio 1: 2). We explore the influence of DESs on the photophysical properties of TO. The fluorescence quantum yield and fluorescence lifetime of TO decreases with increasing temperature due to thermal deactivation. At higher temperature, fluorescence quantum yield of TO decreases in DESs may be due to the molecular rotor nature of TO, with the benzothiazole and quinoline ring of this dye being able to be rotated relative to each other in the excited state. In these solvents, the free volume idea was found to provide a truthful report of the solvent viscosity-temperature behavior, and the probe torsional dynamics. Fluorescence lifetime imaging microscopy (FLIM) was used to insight and observed the distribution of lifetime of TO in the surface of both DESs. The contact angle was determined to show the hygroscopic nature of the DESs. (c) 2021 Elsevier B.V. All rights reserved.

Automated summary

The photophysics and torsional dynamics of thiazole orange (TO) in two deep eutectic solvents (DESs) were studied, with the fluorescence quantum yield and lifetime of TO decreasing at higher temperatures due to thermal deactivation. The molecular rotor nature of TO in DESs may contribute to the decrease in fluorescence quantum yield at higher temperatures. The free volume idea in these solvents accurately predicted the solvent viscosity-temperature behavior and probe torsional dynamics.